General Western Blot Reagents
Abcam 2,3-Butanedione 2-mono x ime (BDM), Non-selective myosin ATPase inhibitor, 500MG
MW 101.1 g/mol. Non-selective myosin ATPase inhibitor (Ki = 5 mM at myosin II). Potent Kv2.1 (DRK1) and L-Type Ca²+ channel inhibitor (Ki and IC₅₀ values are 10.7 nM and 5.8 mM, respectively). Blood-brain barrier permeable. Inhibits muscle contraction.
The product is subject to the following: Abcam Restricted Use Statement
Hach Company Sartorius Biosart 100 Monitor
Sartorius Biosart 100 Monitors, Case of 48 Individually Packaged Monitors. The Sartorius Biosart 100 Monitors are incredibly unique and efficient. By drawing vacuum across the membrane, your sample is quickly and efficiently filtered through the included 0.45 µm filter. When you are done filtering, the monitor quickly converts into its own Petri dish, saving you time and money, while eliminating waste. Simply cap the bottom of the monitor, remove the cylinder side tube, add media, and cap the Petri dish. The Sartorius Biosart 100 Monitors are the most complete, quickest, and easiest to use filtering device that we have seen and are proud to offer this and other high-quality Sartorius branded items.
Sigma Aldrich Fine Chemicals Biosciences Q SepharoseR fast flow Cyt
Q Sepharose(R) fast fLow is a strong anion exchanger based on the well established Sepharose(R) fast fLow ion exchange platform extensively used for preparative protein separations in both research and industrial applications.
Abcam Bradford Reagent, 500ML
Bradford reagent is an improved formulation for protein quantification, offering higher tolerance to detergent contamination and reduced protein-to-protein variation compared to traditional bradford assays. - Cited in over 30 publications - Excellent linearity across a defined range of protein concentrations - Color Change – binds to protein, causing a shift in absorption from 465 nm to 595 nm with a color change from brown to blue
The product is subject to the following: Abcam Restricted Use Statement
LAMDA BIOTECH INC ECL Western Blotting Detection Kit
The ECL Western Blotting Detection Kit contains 100ml of Reagent A and 100 ml of Reagent B solution. Simply mix A and B in a 1:1 ratio right before the application. The mixture is then ready for detection of any immobilized specific antigens in chemiluminescent Western blots through horseradish peroxidase (HRP) labeled antibodies. The kit is designed for excellent sensitivity for protein level as low as femtogram. And the long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the protein signals.
Cell Signaling Technology Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb 100 µl
Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb 100 µl
LICOR NewBlot™ IR Stripping Buffers for NIR Western Blots (100 mL)
NewBlot IR is a robust formulation optimized for near-infrared (NIR) Western blots that works with both nitrocellulose and PVDF membranes. This stripping buffer is a good place to start if you are new to stripping and reprobing NIR Western blots. NewBlot IR works well for removal of all IRDye secondary antibodies, and may be suitable for removal of other dye-conjugated secondary antibodies. NewBlot IR does not require hazardous shipping, unlike many other stripping buffers. Note: NewBlot IR Stripping Buffer is not recommended for loading amounts over 30 µg.
LICOR Revert™ 520 Total Protein Stain for Western Blot Normalization (200 mL)
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. Revert 520 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 520 nm channel for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. The 200 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 500 mL size is sufficient for 50 mini Western blots.
Cosmo Bio Usa Inc CAN GET SIGNAL 250MLCAN GET SI
Accelerator solution for antigen-antibody reactions. Improves sensitivity, specificity, and S/N for antibody blotting, ELISA, etc. For tissue immunostaining use Can Get Signal Immunostain products.
ProteinSimple 66-440 kDa Pre-filled Plates
Compatible with Jess, Abby and Wes systems using Compass v2.7 or higher. Includes 8 Plates (pre-filled with Separation Matrix, Stacking Matrix, Split Running Buffer 3 and Matrix Removal Buffer).
Seracare Life Sciences Inc TMB MEMBRANE ENHANCER
Component of the TMB Membrane Peroxidase Substrate System which allows the substrate to precipitate. TMB Membrane Peroxidase Substrate develops a dark blue stain at the reaction site in the presence of peroxidase-labeled conjugates. The TMB Membrane Enhancer is recommended for use with KPL's 2-compenent TMB Microwell Substrate (Cat. No. 50-76-00) to produce a precipitating substrate.
Seracare Life Sciences Inc SILVER ENHANC KIT-MEMBRANES
KPL's Silver Enhancer Kits are available for use in membrane based applications. This simple yet sensitive system is optimized for use with KPL's gold conjugates for the amplification of gold particles. Silver builds up on the surface of the gold particles as a result of the reduction of silver from one solution to another. Silver Enhancer kits offer low background, sensitivity to the picogram level and slow, controlled development. 15-25 minutes development for microscopy applications.